Silicosis remains a significant global health problem, that results in severe disability. The hallmarks of silicosis are pulmonary inflammation and fibrosis. The model recapitulates acute and chronic phases of the pathology.
Silicosis - Methodology
Species: rats, mice
Silica, single intratracheal instillation
Timeline: in-life phase 4 weeks, total 7 weeks
Silicosis - Readouts & Application
Readouts:
Plethysmography
Pulmonary PV loop analysis
Lung histology with Ashcroft score
Cell count and cytokines in BALF
Collagen and hydroxyproline concentrations
Application:
This model recapitulates the pro-inflammatory, fibrotic and pulmonary physiological manifestations of silicosis, which is essential for comprehensive efficacy and safety assessment of candidate compounds under investigation.
