Carbon tetrachloride (CCl4) exposure in rodents induces liver toxicity, that results in fibrosis. Mouse CCl4 model is the widely accepted because it replicates mechanisms of human pathology.
Species: mice
CCl4, intraperitoneal injection twice a week
Timeline: in-life phase 4 weeks, total 7 weeks
Readouts:
Histology: H&E, PSR
Fibrosis scoring (Ishak scale)
Blood chemistry, ALT, AST, Bilirubin
Liver weight and index
Application:
CCl4 induced liver fibrosis is a well-established and highly reliable model. It is intended to assess anti-fibrotic properties of new drug entities.
