Thrombosis/Anastomosis

Post-surgical accumulation of thrombin, fibrin and platelets limit the success rate of vascular repairs.

Potential antithrombotic compounds are tested for efficacy using this microvascular procedure: a transverse incision through half of the artery is repaired with four interrupted sutures. The two central sutures voluntarily invert a considerable portion of the arterial wall intraluminally, causing significant obstruction of blood flow while maintaining vessel patency.

This procedure produces consistent thrombus formation and the subsequent complete occlusion at the anastomosis if not treated with an antithrombotic agent.

End points:

  • Vessel patency assessment : Milking test + doppler flow monitoring
  • Blood sampling: Platelet count
  • Bleeding time
  • Histology : Light and scanning electron microscope

Rat, Rabbit, Dog