IPST‘s in vitro studies are developed by an experienced team of scientists trained in biochemistry, pharmacology, cellular and molecular biology. Whether GLP-compliant or Discovery-stage, all protocols are customized to fit your needs and to answer any questions you might have on compound efficacy, safety, and toxicity.
With ample cell and tissue culture experience, IPST scientists can isolate sub-cellular components such as mitochondria and various enzymes to monitor an assortment of activities in cell extracts. Methods used include ELISA, mass spectrometry, RT-QPCR, histology and immune-histochemistry (IHC).
IPST‘s experts can measure the effect or identify the mechanism of action of your compound by reproducing physiopathologies in vitro. By submitting selected cell types to specific conditions (examples include: inflammation stimulus, metabolic deficit, or hypoxia), an economical, higher-throughput efficacy model can be validated within a week or two to accelerate your early-phase drug development.
Listed below are a few in vitro models used routinely at IPST:
- hERG-expressing cells lines
- Heterologous ion channel expression systems
- Stem Cells (IPS cell) differentiated into various phenotypes
- Normoxic, hypoxic cardiac and vascular tissues
- Isolated cardiomyocytes (dog, rabbit, rats)
New models are developed every month, as studies are customized for specific applications for IPST clients.