Cardiovascular Muscle Tension

The Cardiac and Vascular Muscle Tension Measurement Assay

Request a Study Outline from IPST

Highlights:

  • Isolated  atrial/ventricular trabeculae & papillary muscles as well as thoracic/abdominal aorta & coronary arteries
  • Isometric tension measurements performed on vertically-mounted tissues
  • Pre-IND (GLP-compliant ) or exploratory designs (non-GLP compliant)
  • Rat, guinea pig, rabbit, dog

Cardiac Muscle Tension measurement : Ventricular and papillary muscle contraction results from a transient depolarization of the myocytes in the heart (action potential). The alteration of the cardiac action potential not only affects the electrophysiology of the organ, but also its mechanical properties. By using contraction-relaxation parameters as an integrated phenomenon, the measurement of the time-course of the contraction-repolarisation cycle in cardiac tissues verifies the effects of a test article on the electrogenic (ion currents) and protein structures involved in its contraction.

Vascular Muscle Measurement : Changes in arterial blood pressure result from variations in the elasticity and tension components of the arterial tree, controlled by exogenous and endogenous factors which fine-tune the tissue’s contractile machinery.  By using  contraction-relaxation parameters as an integrated phenomenon, the analysis of the amplitude and time-course of the contractile cycle in aortic muscle allows the quantification of the effects of a test article on the structures involved in vascular tension, and, indirectly, on arterial blood pressure.

Study outline:

  • Number of tissues exposed to the test article: 5 tissues
  • Number of concentrations of test article tested: 4
  • Stimulation: single or multiple frequencies, electric field stimulation
  • A study includes positive, negative, and vehicle controls

Study outcome:

  • A quantitative assessment A quantitative assessment of the effects of increasing concentrations of a test article on parameters such as amplitude, rates of contraction / relaxation
  • Determination of the EC50 value (if applicable)
  • FDA-ready hard copy  and e-report for electronic IND submission
  • Holistic interpretation of a positive signal, considering all other data generated

Recommended reading:

  1. Allen DG. Kentish JC., The cellular basis of the length-tension relation in cardiac muscle., Journal of Molecular & Cellular Cardiology., 1985; 17(9):821-40.
  2. Manring A. Anderson PA., The contractility of cardiac muscle., Critical Reviews
  3. Lucchesi BR., Role of calcium on excitation-contraction coupling in cardiac and vascular smooth muscle., Circulation., 1989; 80(6 Suppl):IV1-13.
  4. McCall D., Excitation-contraction coupling in cardiac and vascular smooth muscle: modification by calcium-entry blockade., Circulation 1987; 75(6 Pt 2):V3-14