Metabolic screening

Metabolism is an incredibly complex process. Most of the time, a disease’s complications are driven by metabolic disorders which create a “reactive cocktail.” For example, a rise in circulating lipids can accelerate atherosclerosis leading to a phenomenon called “lipotoxicity” and impact many metabolic organs.

IPST’s metabolic screening can help you screen the metabolic impact of your compound in healthy or disease models. Our screening includes:

    • Energy homeostasis – This experiment involves an indirect calorimetry system which allows us to measure the whole body energy expenditure, oxygen consumption, C02 production and respiratory quotient (RQ). This provides valuable information to measure the metabolic state.
      • Indirect calorimetry

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  • Glucose metabolism – Produced mainly by the liver during a fasting period, glucose is used by tissue through an insulin-dependent shuttle. At IPST we can provide rapid evaluation of non-invasive whole-body glucose utilization by a classic glucose tolerance test or via a tissue-specific glucose uptake with radioactivity.
    • Glucose Tolerance Test (GTT) is used to assess the organism’s ability to use glucose as substrate. In humans a GTT is commonly used to diagnose glucose intolerance, insulin resistance or even type 2 diabetes. In animal research, the GTT is used to assess the insulin resistance level and also to evaluate glucose homeostasis. It can also be used to detect the possible side-effects of drugs intended to treat other unrelated diseases. GTT could be performed orally (OGTT) or via an intraperitoneal injection (IPGTT). OGTT mediates real-life glucose utilization with gut-sensing hormone liberation (incretin) as opposed to intraperitoneal evaluation that by-passes the gut tract system. IPGTT is normally used to assess glucose homeostasis independently of hormone secretion. The GTT could serve as a good surrogate of pancreatic function and glucose tolerance.

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    • Insulin Tolerance Test (ITT) is used to assess whole-body sensitivity to insulin. Without affecting the release of insulin by the pancreas, IPST measures the ability of tissues to uptake glucose in response to insulin injection.

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    • Pyruvate Tolerance Test (PTT) is used to assess gluconeogenesis. During a fasting state, hypoglycemia is normally prevented via two hepatic processes: glycogenolysis (the degradation of glycogen) and gluconeogenesis (the generation of glucose from non-carbohydrate carbon substrates including pyruvate and lactate). The Pyruvate Tolerance Test is a variant of the GTT in which pyruvate is injected instead of glucose. The pyruvate bolus will elicit a glycemic excursion that will reflect the hepatic gluconeogenesis.

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